in situ hybridization

Incontinentia pigmenti in a boy with XXY mosaicism detected by fluorescence in situ hybridization

1例荧光原位杂交检测存在XXY镶嵌的男孩出现色素失禁症

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In this paper two patients of antitype stigma has been reported by using G banding techniques of chromosomes and chromosomes in situ hybridization.

本文报告了2例临床症状不典型而用G带分析和染色体原位杂交证实了的该综合征患儿。

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In this paper, several molecular methods such as fluorescence in situ hybridization ( FISH) and denaturing gradual gel electrophoresis ( DGGE) etc.

为此，在介绍变性梯度凝胶电泳（DGGE）以及荧光原位杂交（FISH）等分子生物学新技术的基础上，对这些技术在土壤微生态研究中存在的问题和发展前景做了展望；

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Objective To evaluate the value of a panel fluorescence in situ hybridization ( FISH) in the detection of common chromosome abnormalities in myelodysplastic syndrome ( MDS).

目的评价组合探针荧光原位杂交（fluorescenceinsitehybridization，FISH）在检测骨髓增生异常综合征（Myelodysplastic syndrome，MDS）常见染色体异常中的价值。

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Development of Nov mRNA neurons in central nervous system of the rat in situ hybridization histochemistry and RT PCR study

大鼠中枢神经系统肾母细胞瘤过度表达基因mRNA神经元的发育&原位杂交与RT-PCR研究

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METHODS: The expression and distribution of perforin mRNA in the local infiltrated cells were detected by in situ hybridization using a dig labeled perforin cDNA probe in liver biopsy tissues.

方法：以随机引物法标记人perforincDNA探针，对慢性丙肝患者肝活检标本进行原位杂交，观察肝局部浸润炎性细胞perforin mRNA的表达及分布，分析他们与肝损伤之间的关系。

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Localization of epidemic hemorrhagic fever virus nucleic acid in brain tissue and white blood cells by in situ hybridization

原位分子杂交定位组织细胞中流行性出血热病毒核酸

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In situ hybridization technique of HBV, HEV DNA probe marked by avidin

生物素标记HBV、HEV DNA探针原位分子杂交技术

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Application of Fluorescence in situ Hybridization ( FISH) in the Molecular Biology of Shellfish

FISH技术在贝类分子生物学研究中的应用

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Detection of HCMV DNA in vascular plaques of atherosclerosis by in situ hybridization

原位杂交检测人动脉粥样硬化斑块组织中人巨细胞病毒DNA

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Shiga-like toxin and were not detected with in situ hybridization of radio isotope labeled probe and PCR.

应用同位素标记探针的原位杂交试验及PCR技术均未检查出类志贺样毒素Ⅰ和Ⅱ（SLT－Ⅰ，SLT－Ⅱ）。

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In situ hybridization analysis of mRNA expression of 5-Hydroxytryptamine_4 receptor in intestinal mucosa mast cells

大鼠小肠黏膜肥大细胞5-羟色胺4受体mRNA的表达

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Environmental estrogens screening methods, with VTG mRNA as a biomarker, include real-time quantitative PCR, Northern blot, in situ hybridization.

以VTG mRNA为生物标志物的环境雌激素筛选方法主要包括实时定量PCR、Northern杂交、原位杂交等。

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Live cells were observed under inverted microscope equipped with Hoffman modulation contrast optics, and classified according to their morphology. Chromogenic in situ hybridization ( CISH) using chromosome 17 centromere probe and anti-c-kit immunocytochemistry staining were applied to classified cell smears.

在Hoffman光学镜头下根据细胞形态进行活细胞分类，选取各类细胞分别涂片，行17号染色体着丝粒探针化学显色原位杂交和c-kit抗原表达免疫细胞化学染色。

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Results: Compared to the control group, the NGF like immunoreactivity and signals of NGF mRNA in situ hybridization of depressive group were significantly decreased.

结果：与对照组相比，抑郁组大鼠海马和顶叶皮质神经元NGF免疫阳性颗粒数目减少，着色浅淡；NGF mRNA杂交阳性信号减少。

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Reverse transcription PCR and in situ hybridization were used to detect the expression changes of CaBP mRNA in the brain of PD and control mice.

用反转录PCR及原位分子杂交方法检测PD鼠和正常鼠脑内CaBP mRNA的表达变化。

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A study on chromosome aberration in medical diagnostic X-ray workers by fluorescence in situ hybridization

用荧光原位杂交技术对医用诊断X射线工作者染色体畸变的研究

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The expressions of TGF β 1 and extracellular matrix protein and TGF β 1mRNA were analyzed by immunohistochemistry and in situ hybridization.

用免疫组化及原位杂交观察肾脏TGFβ1表达及细胞外基质（ECM）沉积。以计算机图象分析进行半定量比较。

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Objective To explore the value of interphase fluorescence in situ hybridization ( FISH) in the detection of trisomy 8 in myelodysplastic syndromes ( MDS).

目的探讨间期荧光原位杂交（FISH）技术在检测骨髓增生异常综合征（MDS）8号染色体三体（8三体）中的价值。

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Preparation of digoxigenin labelled probe and detection of HBV DNA in liver and extrahepatic tissue with in situ hybridization

地高辛素标记探针原位杂交法检测肝和肝外组织中乙型肝炎病毒DNA

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Chicken PGCs from a line of White Leghorns were microinjected into Maya duck blastoderms at stage ⅹ. Extragonadal development of donor PGCs in chicken duck chimeric embryos were studied by in situ hybridization with W chromosome specific DNA probes.

通过微注射法将鸡原始生殖细胞（PGCs）注入到鸭的胚盘下腔中，用鸡W染色体DNA探针通过原位杂交的方法对供体PGCs在嵌合体胚胎性腺外的发育作了研究。

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Meanwhile, the citrus chromosome slides used for FISH and rDNA loci situation on chromosome, genetic variation analysis among the homologous tetraploids and diploids, repeated sequence of chromosome in situ hybridization ( CISH) for chromosome identification and species evolvement were discussed.

对用于荧光原位杂交的柑橘染色体制片方法和rDNA在染色体上的分布位置、同源四倍体和相应二倍体的遗传差异分析、重复序列的染色体原位杂交用于染色体识别和物种演化研究等进行了讨论。

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The technique of chromosome in situ hybridization ( CISH) has been extensively applied to genetics and breeding of higher plants.

染色体原位杂交技术近年发展较快，已广泛应用于植物遗传育种实践。

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Methods: Rabbit carotid endothelial injury model was established using Fishman air drying method. Effect of XMT on model rabbits wild-type p53 gene expression was observed by tissue in situ hybridization.

方法：用Fishman空气干燥法，建立家兔颈动脉内皮损伤模型，以组织原位杂交方法观察心脉通对家兔颈动脉内皮损伤后p53基因表达的影响。

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Objective: To identify the deletions in Duchenne/ Becker muscular dystrophy ( DMD/ BMD) by using fluorescence in situ hybridization ( FISH).

目的：应用荧光原位杂交（FISH）筛查技术检测假性肥大型肌营养不良症（DMD/BMD）缺失型携带者。

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Expression of microRNA in human breast cancer detected by in situ hybridization

应用原位杂交技术检测乳腺癌组织中microRNA的表达

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In situ hybridization was used with specific molecular probes to detect the expression of c jun, c fos and c H ras genes during hepatocarcinogenesis in WHV/ myc transgenic mice.

用核酸分子原位杂交的方法，检测了WHV／myc转基因小鼠肝癌发生过程中c－jun、c－fos和c－H－ras基因的表达情况。

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Detection of human papillomavirus DNA of oral squamous cell carcinomas by in situ hybridization

口腔鳞癌组织中人乳头瘤病毒DNA的原位杂交研究

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Objective To explore the differences between radiation-induced translocation and dicentric chromosome aberrations detected by multicolor fluorescence in situ hybridization ( M-FISH) method.

目的探讨用多色荧光原位杂交（M FISH）技术检测的易位和双着丝粒染色体畸变的差异。

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The expression of Nogo protein-A in the brain of MCAO rats was observed by in situ hybridization and the section was subjected to image analysis to determine the mean staining gray.

原位杂交法观察大脑中动脉闭塞大鼠脑内勿动蛋白-A的表达，并对切片进行图像分析，测定染色平均灰度。

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